Vitamin D (25-Hydroxy) Test price comparison
The standard blood test for your vitamin D level. Compare what 6 US providers charge, starting at $46.95.
Also called: 25-hydroxy vitamin D, 25(OH)D, calcidiol · Last updated November 3, 2026
| Provider | Test as listed | Price | |
|---|---|---|---|
| Ulta Lab TestsLowestLab: Quest Diagnostics | Vitamin D 25-Hydroxy Total TestListed on Ulta's Quick Order page | $46.95 | View test → |
| Walk-In LabLab: Labcorp or Quest | Vitamin D, 25-Hydroxy Blood Test | $59.00 | View test → |
| HealthLabsLab: Labcorp or Quest | Vitamin D 25-Hydroxy (Calcidiol) Blood Test | $59.00 | View test → |
| QuestLab: Quest Diagnostics | Vitamin D Test (25-Hydroxy D2 & D3) | $75.00 | View test → |
| Labcorp OnDemandLab: Labcorp | Vitamin D Test | $99.00 | View test → |
| PersonalabsLab: Labcorp or Quest | Vitamin D 25-Hydroxy Blood Test | $135.00 | View test → |
Prices shown are the test price on each provider's page and may not include fees, taxes or promotions. Prices change often, so confirm at checkout. Fees we saw:
- Quest: Adds a $6 physician service fee per order (shown on Quest's product pages).
- Labcorp OnDemand: Check the cart for any added fees.
- Ulta Lab Tests: Ulta's FAQ says a specimen collection (draw) fee, standard $12.95, is added at checkout.
- Walk-In Lab: Adds a $6 physician fee per order (shown on Walk-In Lab's product pages).
- HealthLabs: Check the cart for any added fees.
- Personalabs: Check the cart for any added fees.
What it measures
The 25-hydroxy vitamin D test measures the main circulating form of vitamin D in your blood. It is the standard way to check vitamin D status. Some versions report D2 and D3 separately.
Who orders it
People check vitamin D when a clinician recommends it, when they take supplements and want to see their level, or when they have limited sun exposure.
How to prepare
No fasting is usually required.
Frequently asked questions
Which vitamin D test should I order?
The 25-hydroxy vitamin D test is the standard test for vitamin D status. The 1,25-dihydroxy test is a different, more specialized test.
Do I need to fast for a vitamin D test?
Usually not. Follow the provider's instructions.
Why do vitamin D prices vary so much?
Providers set their own prices for the same lab test. In our November 2026 check, prices for this test ranged from under $50 to over $130.
LaboratoryTests.com is for information only and is not medical advice. Talk with a licensed clinician about which tests are right for you and what your results mean. Medical disclaimer.